• Zeiss Axio Observer inverted microscope
• Yokogawa CSU-10 confocal head
• Lasers: 441, 491, 561, 647 nm
• Objectives: 10X, 25X multi-immersion, 40X oil, 63X oil, 63X water/glycerin, 100X oil
• Hammamatsu 9100 EMCCD camera
• Capabilities: FRAP (405 300mW laser); Photoactivation; FRET
• Software: Volocity acquisition, quantitation, visualization, deconvolution; FRAP modules; Improvision License Server
  

Training and Standard Operating Procedures for WaveFX spinning disc confocal system

• Nikon Ti inverted microscope
  
• 4 Fluorescent Photomuplier Tubes plus a 32 channel spectral detector
  
• Lasers: 405, 457, 477, 488, 514, 561, and 638 nm
  
• Objectives: 10X, 20X multi-immersion, 40X oil, 60X oil and 60X water
  
• Hammamatsu 9100 EMCCD camera
• Capabilities: FRAP, Photoactivation, FRET and spectral unmixing
  
• Software: Nikon Elements 4.2 acquisition, quantitative measurements, deconvolution
  

Training and Standard Operating Procedures for Nikon A1R laser scanning confocal system

• Zeiss Axio Observer inverted microscope
• LSM 880 conventional laser scanning confocal with GaAsP detectors and a spectral detector
  
• Airyscan superresolution detector with fast scan option
  
• 405, 457, 488, 514, 561, 595, and 638 nm lasers
  
• Dry 10x and dry 20x lenses as well as 40x water, 63x oil, 100x oil and 100x TIRF oil lenses
  
• Environmental control chamber for live cell imaging
  
• FRAP, FRET, photoactivation and spectral unmixing capabilities
  
• Image analysis and deconvolution

Training and Standard Operating Procedures for Zeiss Airyscan 

It is the responsibility of every user to inspect the objective lenses and the rest of the microscope and anti-vibration table for oil or other waste. Any problem needs to be noted on the log and the Microscopy Technician notified immediately. Failure to report problems could result in the most recent user on the log to be held responsible. It is the responsibility of every user to check and see if they are the last user of the day. This must be done using the Nikon Confocal calendar on the MBB Facilities web site. Failure to power down the Nikon Confocal overnight will result in disciplinary action.

1st offence: Verbal discussion

2nd offence: Email user and their supervisor plus the Microscopy Oversight Committee and the Chair of MBB

3rd offence: User’s supervisor is charged $100 and the user is notified that they can potentially be banned from use.

4th offence: The user is banned from using the confocal until further notice. 

The confocal acquisition computers are not to be used for data storage. Backup your images at the end of each session and then delete from the acquisition computer hard drive at the soonest possible opportunity. Images not deleted from the acquisition computers can be deleted without notice at any time if needed to create free hard drive space.