Deciphering The Stochastic Kinetics Of Gene Regulation

Synopsis

Gene activity is the prime mover in the living cell, driving a cell’s function at any given time. I will report on recent advances in our ability to describe the stochastic kinetics of gene regulation, achieved through the combination of single-molecule fluorescence microscopy in individual cells, novel image analysis algorithms, and theoretical modeling. We apply our approach to explore gene regulation in a number of organisms representing a gradation of complexity: E. coli bacteria, fly embryos, and mouse embryonic stem cells.