Microscopy equipment

WaveFX spinning disc confocal system (Quorum Technologies)

• Zeiss Axio Observer inverted microscope
• Yokogawa CSU-10 confocal head
• lasers: 441, 491, 561, 647 nm
• objectives: 10X, 25X multi-immersion, 40X oil, 63X oil, 63X water/glycerin, 100X oil
• Hammamatsu 9100 EMCCD camera
• capabilities: FRAP (405 300mW laser); Photoactivation; FRET
• software: Volocity acquisition, quantitation, visualization, deconvolution; FRAP modules; Improvision License Server
• Training and Standard Operating Procedures for WaveFX spinning disc confocal system

Nikon A1R laser scanning confocal system

• Nikon Eclipse Ti inverted microscope
• lasers: 405, 457, 477, 488, 514, 561, 647 nm
• objectives: 10X, 20X multi-immersion, 40X oil, 60X oil
• 4 fluorescent channel PMT detectors and 1 transmitted light PMT detector which can all scan simultaneously
• capabilities: FRAP; photoactivation; FRET; spectral unmixing with spectral detector; hi-speed resonant scanner
• Software: Nikon Elements acquisition software and Nikon Advanced Research analysis software with Autoquant deconvolution module
• Training and Standard Operating Procedures for Nikon A1R laser scanning confocal system
• Appendix to Training and Standard Operating Procedures for Nikon A1R laser scanning confocal system

Funding

This facility has been generously funded by

• Canadian Foundation for Innovation
• British Columbia Knowledge Development Fund
• Molecular Biology and Biochemistry
• Biological Sciences
• Simon Fraser University

Microscopy technician

Tim Heslip
trh2@sfu.ca
778-782-6807 office
Mon-Fri 9:30-4:30pm
availability schedule

Location of Facility

The facility is situated in room SSB7145 of the South Science Building (directions).

Useful Microscopy Resources

• Fluorescence SpectraViewer from Invitrogen to plot and compare spectral profiles of different fluorophores
• Alternate spectral viewer
• additional resources: Nikon confocal support
• Improvision (PerkinElmer) website for information on the Volocity software
• A1Rsi confocal microscope simple operation manual

Microscopy oversight committee

Please feel free to contact the oversight committee with suggestions for improving the Facility.

• contact Dr. Leroux
• list of committee members

Policies

All users, please read carefully.

• Microscopy users policy