Table 1: Standard curve _________________________________________________ Amount Amount H2O Absorbance of Stock of protein at 595 nm _________ __________ ____ [µl] [µg] [µl] _________________________________________________ 0 blank 800 5 795 10 790 20 780 30 770 40 760 _________________________________________________ Table 2: Protein concentration during enzyme purification ____________________________________________________________________ Samples: dilution Volume [µl] A595 protein original conc. [µg] [mg/ml] [mg/ml] ____________________________________________________________________ HOM P1 SN1 P2 SN2 P3 SN3 SN4 _____________________________________________________________________ back