Subcloning lab results
Plate count results (From Group 12 on Thursday)
|
Plate |
Blue colonies |
“White” colonies |
|
pPBH plasmid miniprep |
0 |
52 |
|
mock ligation |
0 |
0 |
|
unligated digested plasmid |
0 |
0 |
|
test ligation (40 µl) |
15 |
3 |
|
test ligation (10 ul) |
2 |
1 |
|
control ligation (40 ul) |
49 |
0 |
|
control ligation (10 ul) |
8 |
0 |
These are typical results for a ligation using vector that has not been phosphatased.