BISC 431 Molecular Biotechnology
Course Outline
(2002)
Instructor: Dr. Robert W. Sharp Rob Sharp
Office: B9232
E-mail: rwsharp@sfu.ca
Phone: 604 291 5626
Lectures: Tuesday 10:30-11:20, Room C9000
Thursday 9:30-11:20, Room C9000
Labs: Tuesday, Wednesday, Thursday, 13:30-17:20, SSB 8133
Office Hours: Tuesday 11:30-12:20, Wednesday 10:00-11:00, Thursday 11:30-12:30
Description: Laboratory/Lecture molecular biotechnology course
Textbook: Howe, C. Gene Cloning and Manipulation
additional materials will be placed on reserve in the library
Lab experiments:
Plasmid and genomic DNA preps, recombinant DNA techniques, Southern blots, PCR, sequencing, and sequence analysis will be performed in lab exercises. Some labs may extend for more than one lab period, and may require participation outside of the scheduled lab period. Study questions will be included in lab handouts answers to these will be due during the next lab period. A full lab report on the terms experiments will be due in the final week of classes. Students will also be expected to keep an up-to-date, neat, lab book (dated and written in pen). Lab books will be requested at random intervals, without prior warning.
Lab Schedule (Titles of labs will be converted to
hyperlinks to lab handouts)
Week |
Dates |
Lab |
1 |
Tues. Jan. 15-Thurs. Jan. 17 |
|
2 |
Tues. Jan. 22-Thurs. Jan. 24 |
|
3 |
Tues. Jan. 29-Thurs. Jan. 31 |
|
4 |
Tues. Feb. 5- Thurs. Feb. 7 |
|
5 |
Tues. Feb. 12-Thurs. Feb. 14 |
|
6 |
Tues. Feb. 19-Wed. Feb. 20 |
no labs reading break |
7 |
Tues. Feb. 26-Thurs. Feb. 28 |
|
8 |
Tues. Mar. 5- Thurs. Mar. 7 |
|
9 |
Tues. Mar. 12-Thurs. Mar. 14 |
|
10 |
Tues. Mar. 19-Thurs. Mar. 21 |
|
11 |
Tues. Mar. 26-Thurs. Mar. 28 |
|
12 |
Tues. Apr. 2- Thurs. Apr. 4 |
Full lab report due
Lab
report format |
Aseptic technique and equipment use Chemical and radiation safety
Lecture topics:
Lecture topics will mostly be related to laboratory material. Topics covered will include recombinant DNA techniques, PCR, gene expression, and mutagenesis. A library research project will also be due in the first week of March.
Lecture notes:
(Click on links below for lecture notes)
(These are outline notes only
supplement with notes in lectures and text readings)
Preparing DNA for cloning overview
vectors plasmids
λ vectors introduction
vectors for cloning large DNA fragments
preparation of large DNA fragments
preparation of foreign DNA restriction enzymes
preparation of probes oligonucleotide synthesis
screening Southern blots
probes
cloning ligation and transformation
gene analysis and mapping restriction mapping
sequencing longer sequences genomic sequencing strategies
Grading:
|
Date |
Time |
Percent of final grade |
Midterm |
February 14 |
10:30 |
15 % |
Final |
Friday, April 12 |
8:30-11:30 |
50 % |
Lab study questions |
various |
17:20 |
8 % |
Lab note book |
various |
13:30 |
2 % |
Full lab report |
April 2-5 |
17:20 |
15 % |
Library research project |
~ March 5 |
10:30 |
10 % |